A rapid troponin test (blood, serum or plasma) is an immunochromatographic assay for the qualitative determination of cardiac troponin I and its complex in serum, plasma or human whole blood at a concentration equal to or greater than 1 ng / ml. It is intended for use as a screening test by professionals, as an adjunct to the diagnosis of acute myocardial infarction (MI).
The combined OnSight Express test for the detection of troponin I can only provide preliminary analysis results to help with the diagnosis of HPI. Any use or interpretation of this preliminary test result should also depend on the symptoms of the patient and the professional judgment of the physician. A more precise test method, such as an instrumental method, should be used to confirm the test result obtained with this device.
Cardiac troponin I (cTnI) is a protein that is found in the heart muscle and has a molecular weight of 22.5 kDa. Along with troponin T (TnT) and troponin C (TnC), troponin I (TnI) forms a complex of troponin in the heart, which plays a fundamental role in the transmission of intracellular calcium signals, triggering the interaction of actomyosin1. Human cTnI has additional amino acid residues at its N-terminus that do not exist in skeletal forms, thus making cTnI a specific cardiac marker 1-3.
As a rule, the level of cTnI in the blood is very low. cTnI is released into the bloodstream in the forms of free cTnI and cTnI-C-T complexes in 4-6 hours after damage to myocardial cells 2,4,5. An elevated cTnI level can reach 50 ng / ml for 60-80 hours after GI and can be detected within 10-14 days after GIM 2,4,5. Thus, circulating troponin I is a specific and sensitive marker for the diagnosis of AMI.
The combined OnSight Express Test for the detection of troponin I is designed to detect elevated troponin I and its complex in serum, plasma, or human blood in less than 15 minutes with minimal qualified personnel without the need for laboratory equipment.
The combined OnSight Express Test for Troponin I is a chromatographic immunoassay. The test cassette consists of: 1) pads for a bard conjugate containing monoclonal antibodies against troponin I that are coupled with colloidal gold (antibody conjugates); 2) a nitrocellulose membrane strip containing a test line (strip T) and control line (strip C). The strip T is pre-coated with other antibodies against troponin I, and strip C is pre-coated with the antibody against the control line.
When sufficient volume of the test sample is placed in the well for the test cassette samples, the sample migrates under the action of capillary forces through the cassette. The elevated levels of troponin I, if present in the sample, will interact with antibody conjugates. The immune complex is then fixed to a membrane previously coated with anti-troponin I antibodies, forming a T-band barrier, indicating a positive test result for the detection of troponin I.
The absence of a T strip indicates a negative result. The test contains an internal control (strip C) that should display the bronchial line of the immunocomplex of the antibody conjugates to the control line, regardless of the presence of troponin I in the sample. If the strip C does not develop, the test result is invalid and the sample must be retested by another device .
A rapid troponin test, price, buy, ukraine
- Brands CTK Biotech, США
- Product Code: 1 шт
- Availability: In Stock