Lactobacagar - the differential nutrient medium that stimulates the growth of lactobacilli and inhibits extraneous microflora. The nutritional part of the environment consists of complex organic components (peptone, yeast extract), carbohydrate (glucose) and mineral supplements (magnesium, manganese). The precise composition optimal provides "picky" Lactobacillus nutrients in the required concentrations. When the salts of organic acids (acetate, citrate), and low pH inhibit the growth of extraneous microorganisms. This makes it possible to determine the presence of Lactobacillus in such planted samples as dairy and foods, or defecation. Samples cultured at 37 ° C for 48 hours to hold dishes with gasparatos providing the concentration of carbon dioxide 5-10%.
Purpose: Cultivation of Lactobacillus
Principle of action: Reduced pH of the medium suppresses the growth of the concomitant microflora and is optimal for the development of lactobacilli. acetate and sodium citrate inhibit the growth of the fungal flora. Magnesium chloride, manganese sulphate and ascarbic acid stimulate the growth of lactobacilli
Peptone enzymatic - 15 g / l
Glucose - 15 g / l
Agar microbiological - 10 g / l
Sodium acetate - 4 g / l
Yeast extract - 2.8 g / l
Potassium dihydrogen phosphate - 1.5 g / l
Ammonium citrate - 1.5 g / l
Magnesium chloride - 0.1 g / l
Ascorbic acid - 0.04 g / l
Manganic sulfate - 0.04 g / l
Instructions for use:
1. Dissolve 50 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Autoclate for 15 min at 121 ° C
4. Cool to 50-45 С and pour into sterile Petri dishes
PH level: 6.0-0.2 ° at 25 ° C
Delivery time: 5 d.
Lactobacagar, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days