The medium is intended for accumulation and identification of the growth of coliform bacteria. The growth of microorganisms is accompanied by a change in color from green to yellow, often with the release of gas. Carbon source in the medium is lactose, therefore it can be considered partially selective. Lactose negative bacteria can also evolve due to other complex organic components, but exceptional conditions of accumulation are only bacteria capable of fermentation of lactose.
Purpose: Accumulation and identification of coliform bacteria
Principle of action: In the environment contains only one carbohydrate - lactose. Bacteria that do not ferment lactose develop in the environment without changing the color. Lactose-fermenting microorganisms change the color from green to yellow. Placing in the environment of glass shelves can be found in the formation of gas.
The peptone is enzymatic - 7 g / l
Lactose - 5,5 g / l
Sodium chloride - 4 g / l
Yeast extract - 0.5 g / l
Bromotymol blue - 0,04 g / l
Instructions for use:
1. Dissolve 17 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Spray 10 ml in glass flask test tubes and autoclave for 15 minutes at 110 ° C
PH level: 7,1-0,2 at 25 ° C
laktose peptone broth, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days