Nutrient medium intended for generation of the agents of enteric yersiniosis and pseudotuberculosis. The growth of gram-positive and gram-negative microflora is inhibited. Escherichia coli and Shigella become yellow, via the fermentation of glucose. Yersinia turns yellow within the first 24 hours, as they are also fermented glucose. On the second day of incubation Yersinia breaks down urea and their colonies take on a blue color. Other urea positive microorganisms acquire blue color, slippery, indistinct colonies.
Purpose: Isolation of intestinal yersiniosis and pseudotuberculosis
Principle of action: The growth of gram-positive and partially gram-negative microflora is inhibited. E. coli and shigella acquire yellowish color, due to the fermentation of glucose. Yersinia yellow for the first 24 hours, as they also ferment glucose. On the second day of the incubation of Yersinia, urea is cleaved and their colonies become blue.
The peptone is enzymatic - 17.85 g / l
Glucose - 10 g / l
Agar microbiological - 12 g / l
Sodium chloride - 3 g / l
The bile is 4 g / l
Urea - 5 g / l
Bromotymol blue - 0,128 g / l
Diamond green - 0,00033 g / l
Instructions for use:
1. Dissolve 52 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Cool to 50-45 С and pour into sterile Petri dishes, dry by adhering to the rules of asepsis.
PH level: 7.6-0.2 ° at 25 ° C
Delivery time: 5 d.
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Information updated: 07/24/2018
Yersinia medium, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days