Serum Escherichia coli, 2 ml, DENKA SEIKEN, Japan

Escherichia coli is a group of gram-negative sticks belonging to the Enterobacteriaceae family and is considered one of the bacteria of the normal intestinal microflora of humans. Their serological types are determined by a combination of a somatic antigen (O group: O1-O173) and flagella (H type: H1-H56) antigens.

E. coli causing intestinal infectious diseases, including diarrhea, acute gastritis or colitis, are pathogenic E. coli and are divided into the following 4 groups according to differences in pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), and enterohemorrhagic E coli (EHEC). Although the identification of pathogenic E. coli requires testing their pathogenicity, pathogenic E. coli often have specific serotypes; consequently, the definition of serogroup and serotype is necessary for the screening of pathogenic E. coli.

O and H serum are liquid products containing specific agglutinins for E. coli serotyping. O-group of sera is prepared by hyperimination of healthy pigs (polyvalent serums) or healthy rabbits (monovalent serums) with reference strains of microorganisms of each serotype, inactivated by formalin, heated at 56 ° C for 30 minutes. Cross-agglutinins are removed by absorption and antiseptic filtration. For the preparation of H-sera, healthy rabbits were immunized with E. coli flagella. Group O-serums are used for the O-serotipation test by agglutination on the glass, and H-sera for H-serotyping by agglutination in the test tubes.

 PRODUCT

1. O-group serums (set l)

These are liquid products containing specific somatic (O) antibodies (polyvalent serums: pork, monovalent serums: rabbits) to microorganisms and 0.08% sodium azide as preservative.

  2 ml x 51 bottle (8 vials of polyvalent serum, 43 vials of monovalent serum)

Polvalent serum

Monovalent

Polvalent 1 О1, О26, О86a, О111, О119, О127a, О128

Polvalent 2 О44, О55, О125, О126, О146, О166

Polvalent 3 О18, О114, О142, О151, О157, О158

Polvalent 4 О6, О27, О78, О148, О159, О168

Polvalent 5 О20, О25, О63, О153, О167

Polvalent 6 О8, О15, О115, О169

Polvalent 7 О28ac, О112ac, О124, О136, О144

Polvalent 8 О29, О143, О152, О164

2. H serum (kit 2)

These are liquid products containing flagella (H) antibodies (rabbit) of microorganisms and 0.08% sodium azide as preservative.

 2 ml x 22 vials (22 vials of monovalent serum)

H-serum H2, H4, H5, H6, H7, H9, H10, H11, H12, H16, H18, H19, H20, H21, H27, H28, H34, H40, H41, H42, H45, H51

3. Group O-Serum (Alternative)

Polyvalent II (alternative) - content: О26, О55, О111,0119, 0126

Polyvalent III (alternative) - content: О86, О114, О125,0127,0128

Polyvalent IV (alternative) - content: О44, О1212, О124,0142

 NECESSARY USE

Determination of serotypesE. coli

 PRINCIPLE OF DEFINITION

When this reagent is mixed with E.coliis strain with an antigen corresponding to the reagent, the antigen-antibody reaction causes agglutination formation. The reaction is taken into account macroscopically to determine each serotype.Delivery time: 5 days

Information updated: 02.03.2018