Lefson's Selenium Broth is a liquid nutrient medium for the accumulation of salmonella. Selenite inhibits the development of most bacteria, but does not inhibit the development of salmonella. The peculiarity of the medium is the speed of the analysis, selective accumulation is carried out within 6 hours. The key factor in the work environment is the pH level. Mannit is added to the medium to maintain acidic pH, from which microorganisms form an acid.
Purpose: Selective environment for the accumulation of salmonella.
Principle of action: Selenite suppresses the development of most bacteria, except salmonella, due to which selectivity is achieved. Manit is a source of carbohydrates. The buffer system maintains a constant pH level.
The peptone is enzymatic - 5 g / l
Manite - 4 g / l
Sodium hydrophosphate - 7,5 g / l
Sodium dihydrogen phosphate - 7.5 g / l
Sodium selenite - 4 g / l
Instructions for use:
1. Dissolve 28 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved, poured into sterile tubes.
3. Do not autoclate
PH level: 7,0-0,2 at 25 ° C
Delivery time: 5 d.
Information updated: 02.03.2018
Selenite broth leifson, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days