Salt agar contains a high concentration of sodium chloride. In such quantities, chloride inhibits the growth of the concomitant microflora, but does not affect the development of staphylococci, which contributes to its excretion from the clinical material. In addition, the nutrient part of the medium fully supports the development of lecithin staphylococci, and can be determined by the formation of a rainbow zone around colonies.
Purpose: Staphylococcal isolation and differentiation by the ability to produce lecithinase
Principle of action: High concentration of sodium chloride inhibits the growth of most bacteria, but does not affect the growth of staphylococci. Also, colonies that produce lecithinase form an iridescent area around them, which is visible when the light falls at an angle.
The peptone is enzymatic - 10 g / l
Sodium hydrophosphate - 0.5 g / l
Agar microbiological - 15 g / l
Sodium chloride - 70 g / l
Yeast extract - 4.5 g / l
Instructions for use:
1. Dissolve 100 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Autoclate for 20 minutes at 121 C
4. Cool to 50-45 ° C and pour into sterile petty cups.
PH value: 8.0-0.2 ° at 25 ° C
Delivery time: 5 d.
Information updated: 02.03.2018
Salini staphylococcus agar, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days