Russel medium is used for the differentiation of enterobacteria on the ability to ferment lactose or glucose. According to the analysis, the bacteria are divided into three groups: lactose fermenting, glucosaminidase and bacteria, not fermenting carbohydrates. Analyze samples after 19-20 hours, because earlier they can be incorrect, because of a staged fermentation medium components. Be sure to leave one control vial (original color is green).
Purpose: Differentiation of enterobacteria by the ability to ferment lactose or glucose.
Principle of action: In the process of fermentation of glucose, the medium becomes yellow. After the end of glucose, microorganisms develop due to proteins that color the slitted part of the agar in a blue color. Bacteria capable of fermenting glucose and lactose, staining the whole environment in yellow. Microorganisms that consume only proteins, painted the beveled part of the agar in blue, with the environment of the column green.
The peptone is enzymatic - 10 g / l
Lactose - 10 g / l
Agar microbiological - 10 g / l
Sodium chloride - 4 g / l
Glucose - 1 g / l
Yeast extract - 1 g / l
Bromotymol blue - 0,03 g / l
Instructions for use
1. Dissolve 36 g of medium when heated in 1 liter of purified water.
2. Boil for 2-3 minutes, until the components are completely dissolved.
3. Pour into test tubes and autoclaving at 112 ° C for 20 minutes.
PH level: 7.3-0.2 ° at 25 ° C
Delivery time: 5 d.
The information is valid: 04/23/2018
Russel medium, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days