This environment is used for the differentiation of Enterobacteriaceae by the ability to deaminate phenylalanine. For the analysis of the environment is prepared in the form of the slanted agar in test tubes. The research culture is inoculated and cultured at 37 ° C for 24 hours. At the end of cultivation in test-tube add ferric chloride solution, and the mixture turns green in color. In the process of growth of a culture which is able to deamination phenylalanine ammonia which is formed when additions of iron ions to form a green complex.
Purpose: Differentiation of microorganisms by the ability to disinfect phenylalanine.
Principle of action: After cultivating bacteria on a medium, in the form of a sloped agar, 2-3 drops of 10% solution of chlorine iron are added to the test tube. Changing the color of the green indicates the ability of the bacteria to deaminate phenylalanine. In the absence of a change in the color of the green, the deamination did not take place.
Agar microbiological - 10 g / l
Sodium chloride - 5 g / l
Yeast extract - 3 g / l
Sodium hydrophosphate - 1 g / l
Fenilalanine - 1 g / l
Instructions for use:
1. Dissolve 20 g of medium when heated in 1 liter of purified water.
2. Boil for 2-3 minutes, until the components are completely dissolved, pour the test tubes (smooth without a column)
3. Autoclate for 10 minutes at 121 C.
PH level: 7.3-0.2 ° at 25 ° C
Delivery time: 5 d.
The information is valid: 04/23/2018
Phenylalanine agar, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days