Kessler's medium is used to detect E. coli in the sample. This is achieved through two factors of the nutrient medium. First, the presence of bile suppresses the concomitant non-pathogenic microflora, the crystalline violet suppresses gram-positive bacteria, and lactose as the only source of carbon suppresses bacteria that can not ferment it. Secondly, the use of a glass float allows us to clearly identify the presence of coliforms on the basis of gas release.
Purpose: Detection of coliform bacteria
Principle of action: The selective properties of the Kessler environment are due to bile and crystalline violet. The only carbohydrate in the environment - lactose. With the help of a glass float, it is possible to detect the release of gas characteristic of coliform bacteria.
Lactose - 10 g / l
The peptone is enzymatic - 10 g / l
The bile is 3 g / l
Crystal violet - 0.015 g / l
Instructions for use:
1. Dissolve 23 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Pour into glass flask test tubes and autoclave for 20 minutes at 112 C
PH level: 7.5-0.2 ° at 25 ° C
Delivery time: 5 d.
Information updated: 02.03.2018
Nutrient medium Kessler, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days