Diamonds green agar are used for the selective isolation of salmonella (other than Salmonella typhi) from faeces and other
materials; It is also recommended for the study of food products, including dairy products.
Composition g / l
Peptone Proteasic 10.00
Yeast Extract 3.00
Sodium Chloride 5.0
Diamond Green 0,0125
Phenol red 0.08
A bottle of 500 g of dry medium.
Method of cooking:
Mix 50.1 g of dry medium in 900 ml of distilled water. The mixture is boiled to the full
dissolving ingredients. Bring the volume of the liquid to 1000 ml, check the pH and, if necessary,
adjust the value to 6.9 ± 0.2, using a 10% solution of NaOH or a 10% solution of HCl.
Sterilize in an autoclave at 121 ° C for 15 minutes.
Density of the finished medium:
The medium corresponding to the density of 1.2% agar gel is formed.
Color and transparency of the finished medium:
The finished medium has a green-brown color, slightly opalescents, if in Petri dishes
gel is formed.
Acidity of the medium:
At 25 ° C, an aqueous solution (5.0% w / v) pH 6.9 ± 0.2.
Principle and score of the result:
Used to determine Salmonella in mixed flora by inhibition
species of E. coli, Proteus and Pseudomonas.
Peptone provides the necessary amino acid and chemical compounds with nitrogen.
Diamond green suppresses most bacteria, gram-positive and gram-negative.
Phenol red is an indicator of acid-base balance.
After inoculation of the medium by the test sample, place it in the incubator on
18-24 hours at a temperature of 35-37 ° C.
Microorganism Streams (ATCC) Rising Color
Escherichia coli (25922) is absent -
Salmonella Typhimurium (14028) abundant pink-red
Proteus Vulgaris (13315) is a bad pink
Salmonella Enteritidis (13076) abundant pink-red
Pseudomonas aeruginosa (10145) is a bad pink
Diamonds green agar, 500 gr
- Brands Micromaster, Индия
- Product Code: 500 гр
- Availability: 2-3 Days