Agar for clostridia is used to cultivate and count the species of Clostridium and other anaerobes.
Composition g / l
Casein Hydrolyze 10.00
Meat extract 10,00
Yeast Extract 3.00
Sodium Chloride 5.00
Sodium acetate 3,00
L-Cysteine Hydrochloride 0.50
Agar 10.00 13.50
A bottle of 500 g of dry medium.
Method of cooking:
Mix 51.00 g of dry medium in 900 ml of distilled water. The mixture is boiled to the full
dissolve the ingredients, check pH and, if necessary, adjust the value of 6.8 ± 0.2,
using 10% NaOH solution, or 10% solution. HCl.
Sterilize in an autoclave at 121 ° C for 15 minutes.
Density of the finished medium:
The medium responsible for the density of 1.35% agar gel is formed.
Color and transparency of the finished environment:
The finished medium has a light yellow color, slightly opalescents, if formed in Petri dishes
Acidity of the medium:
At 25 ° C, an aqueous solution (5.10% w / v) pH 6.8 ± 0.2.
Principle and score of the result:
Used to study spore-forming anaerobes, in particular Clostridium
butiricum, for calculating clostridia by dilution in test tubes or for
preparation of cups for the separation of clostridia.
Other spore-forming anaerobes, streptococci, and also grow on these environments
Lactobacillus., the environment has a rich composition, but is not selective.
Casein Hydrolyzate, Meat and Yeast Extracts, Starch, L-Cysteine and Sodium Acetate
ensure the presence of the nutrients necessary for the growth of clostridia.
Glucose is a fermenting substrate. Sodium chloride provides the optimum
osmotic pressure. The medium can be provided selectively by adding 15-20 mg
mg (per liter of medium) of polymyxin B.
After inoculation of the medium by the test sample, place it in the incubator on
48 hours at a temperature of 35-37 ° C.
Microorganism Streams (ATCC) Growth
Bacteroides fragilis ATCC (25745) abundant
Bacteroides vulgatus (8482) abundant
Clostridium perfringens ATCC (13124) abundant
Clostridium butyricum (9690) abundant
Agar for clostridia, 500 gr
- Brands Micromaster, Индия
- Product Code: 500 гр
- Availability: 2-3 Days