Nutrient agar is a general medium used for a wide range of microorganisms.

Purpose:

Nutrient agar is used for less demanding microorganisms, as well as for permanent crops (general medium). The medium contains beef extract, yeast extract and peptone as a source of nitrogen. Sodium chloride guarantees osmotic balance. Agar is an agent. The addition of 10% of various biological fluids, such as blood, serum and egg yolk makes it suitable for growing the relevant demanding organisms. For example: Pseudomonas aeruginosa produces a blue-green pigment, piocyanin, which diffuses into the medium, giving a strip of characteristic color. To grow a specific bacterial culture, spread bacteria on the plate with a sterile tampon or a modified loop. Bacteria will grow and become visible within 24 - 48 hours. It is usually used in the laboratory when preparing stocks of bacterial cultures.

Composition:

Agar - 15.00 Gram / liter

Peptone - 5.00 Grams / liter

Sodium chloride - 5.00 Grams / liter

Beef Extract - 1.50 Gram / liter

Yeast extract - 1.50 gram / liter

Cooking method:

Dissolve 28 g of powder in 1000 ml of distilled water. Carefully heat to boiling with gentle stirring until the medium dissolves completely. Sterilize by autoclaving at 15 atm (121º) for 15 minutes. Cool to 45-50 ° C before use.

pH (at 25 ° C): 7.4 ± 0.2

Delivery time: 5 p. D.