Description
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Agar for clostridia is used to cultivate and count the species of Clostridium and other anaerobes.

Composition g / l

Casein Hydrolyze 10.00

Meat extract 10,00

Yeast Extract 3.00

Dextrose 5.00

Sodium Chloride 5.00

Sodium acetate 3,00

Starch 1.00

L-Cysteine Hydrochloride 0.50

Agar 10.00 13.50

Appearance:

A bottle of 500 g of dry medium.

Method of cooking:

Mix 51.00 g of dry medium in 900 ml of distilled water. The mixture is boiled to the full

dissolve the ingredients, check pH and, if necessary, adjust the value of 6.8 ± 0.2,

using 10% NaOH solution, or 10% solution. HCl.

Sterilize in an autoclave at 121 ° C for 15 minutes.

Density of the finished medium:

The medium responsible for the density of 1.35% agar gel is formed.

Color and transparency of the finished environment:

The finished medium has a light yellow color, slightly opalescents, if formed in Petri dishes

gel.

Acidity of the medium:

At 25 ° C, an aqueous solution (5.10% w / v) pH 6.8 ± 0.2.

Principle and score of the result:

 Used to study spore-forming anaerobes, in particular Clostridium

butiricum, for calculating clostridia by dilution in test tubes or for

preparation of cups for the separation of clostridia.

 Other spore-forming anaerobes, streptococci, and also grow on these environments

Lactobacillus., the environment has a rich composition, but is not selective.

 Casein Hydrolyzate, Meat and Yeast Extracts, Starch, L-Cysteine and Sodium Acetate

ensure the presence of the nutrients necessary for the growth of clostridia.

 Glucose is a fermenting substrate. Sodium chloride provides the optimum

osmotic pressure. The medium can be provided selectively by adding 15-20 mg

mg (per liter of medium) of polymyxin B.

 After inoculation of the medium by the test sample, place it in the incubator on

48 hours at a temperature of 35-37 ° C.

Results:

Microorganism Streams (ATCC) Growth

Bacteroides fragilis ATCC (25745) abundant

Bacteroides vulgatus (8482) abundant

Clostridium perfringens ATCC (13124) abundant

Clostridium butyricum (9690) abundant

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Agar for clostridia, 500 gr

Brands Micromaster, Индия
Product Code: 500 гр
Availability: 2-3 Days

2,800.00 грн

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Agar for clostridia, 500 gr

2,800.00 грн

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