Nutrient broth - basic liquid nutrient medium. Supports the growth of most bacteria and has no inhibitory action; uses to the accumulation of biomass and study of metabolic products of the bacteria. The broth fully meets the needs of microorganisms for nutrients and supports the development and production of characteristic compounds.
Purpose: Accumulation of microorganisms
Brief description and explanation: At the beginning of the 19th century, the American Association for Health (APHA) proposed a formula for Pure Agar as a standard culture medium used for water testing. The nutrient broth has the same formula as nutritious agar, with the exception of agar. Nutrient agar is the main nutrient medium used to support the growth of microorganisms, the cultivation of demanding organisms in the case of enrichment with the serum or blood, and nutrient agar used to check the purity of the culture before conducting biochemical or serological testing of the culture. The nutrient broth is used as a pre-enrichment medium when testing certain foods and dairy products for the presence of Salmonella spp. Salmonella can be present in dried or processed foods in sublethal doses or in small amounts. The presence of other bacteria and components of a food sample may interfere with the growth and recovery of bacteria of the genus Salmonella. Pre-enrichment of nonselective media such as nutrient broth allows damaged cells to regenerate, reduce the content of toxic or inhibitory substances, and also provides the advantage of Salmonella growth compared to other bacteria. Nutrition broth is included in many standard methods for testing food, dairy products and other materials. The nutrient broth may be enriched with other ingredients, such as carbohydrates, blood, serum, egg yolk, etc., to make it suitable for growing the relevant microorganisms.
Principle of action: Peptones from animal tissues, beef extract and yeast extract are the source of the necessary compounds of nitrogen, carbon, vitamins and some trace elements, necessary trace for growth of bacteria. Sodium chloride maintains the osmotic balance of the environment.
The peptone is enzymatic - 10 g / l
Sodium chloride - 4.5 g / l
Yeast extract - 0.5 g / l
Instructions for use:
1. Dissolve 28 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Autoclate for 15 min at 121 ° C
PH level: 7.2-0.2 ° at 25 ° C
Delivery time: 5 d.
The information is relevant to: 03.02.2018
Nutrient broth, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days