Blykfeldt's medium is used to accumulate lactic acid bacteria and to identify their growth.
Blykfeldt's medium is a liquid nutrient medium characterized by high growth qualities, which promotes the rapid development of lactic acid bacteria. Inhibitors and selective agents are absent, so a clean culture is needed for the work.
The bromocresol purple indicator detects the formation of acids (the products of the life of bacteria) by changing the color from violet to yellow.
The cultivation time is 48 hours at 37 ° C.
Appointment Blickfeldt Environment: Cultivation of lactic acid bacteria
Principle of action The Blykfeldt medium: A liquid nutrient medium, with an indicator of bromocresol purple. Ensures the development of lactic acid bacteria, which is accompanied by a change in the color of the indicator from violet to yellow, due to the accumulation of acids. The selective effect does not have a medium; therefore, a pure culture is required for the work.
Composition of the Blykfeldt Environment:
Glucose - 10 g / l
Lactose - 10 g / l
The peptone is enzymatic - 5 g / l
Yeast extract - 4 g / l
Bromocresol purple - 0.01 g / l
Instructions for using the Blykfeldt environment:
1. Dissolve 29 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until complete dissolution of the components, pour into the appropriate utensil.
3. Autoclaving for 20 minutes. at a temperature of 117 C.
PH level: 7.3-0.2 ° at 25 ° C
Medium bleeckfeldts, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days