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  • Fraser Broth Base, 500 gr

Fraser Broth Base


Fraser's broth (DM1292) is recommended for primary and secondary enrichment, selection and calculation of Listeria monocytogenes in food and animal feeds.

Brief description and explanation:

Listeria are microaerophilic, gram-positive, asporogenic, non-encapsulated, unbranched, regular, short, moving sticks. Mobility is most pronounced at 20 ° C. The most commonly encountered bacteria found in potentially potentially Listeria sources are: streptococci, especially enterococci, micrococci, Bacillus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris. (1) Listeria grows in the range of pH 4.4-9.6 and survives in food products with a pH level outside these variables. (2) The definition of Listeria is based on the successful excretion of the organism, biochemical characteristics and serologic confirmation.

Among Listeria, only Listeria monocytogenes are known as a pathogen that causes human infectious diseases. In 1926 Murray, Webb and Swann (3) first described that Listeria monocytogenes is a widespread public health and food industry problem. This organism can cause diseases of people, such as meningitis, encephalitis and septicemia; the tropism of Listeria monocytogenes to the central nervous system leads to serious illnesses, often with high lethality or with neurological disorders, as complications after the disease, (4) especially in attenuated individuals and pregnant women. (5) The first food outbreak of listeriosis was reported in 1985 . (6) Since then, microbiological and epidemiological data on sporadic and epidemic cases of listeriosis have shown that the main pathway for the transmission of listeriosis is through the consumption of foods contaminated with Listeria monocytogenes. (7) which can be sources of contamination, include Mexican cheese, cabbage salad, turkey sausages, pasteurized milk and pickled pork tongue. (8) Listeria monocytogenes were isolated from dairy and other food industries; In addition, Listeria monocytogenes is widespread in nature, present in many unprocessed products, in soil, sewage, silage and river water. (9)

The basis of the Fraser broth is based on the formula Fraser and Sperber (10) and is used to detect listeria in foods. Fraser's broth is designed to provide optimum conditions for Listeria's growth of food and environmental samples.

Principle of action:

Fraser's broth contains peptic germ of animal tissue, casein hydrolyzate, yeast extract and meat extract that make the environment highly nutritious by providing nitrogen, carbon and other nutrients essential for the growth of organisms. Phosphates provide buffering properties of the medium. All types of listeria demonstrate beta-glucosidase activity, which is proved by blackening of the medium. Listeria hydrolyze escutaneous glucose and esculental. The latter, in combination with the trivalent iron ions from trivalent ammonium citrate (MS130), forms 6-7-dihydroxycumarine, a black-brown complex. Ammonium citrate of iron also increases the growth of L. monocytogenes. High salt tolerance of listeria (to sodium chloride) is used to inhibit the growth of enterococci. Lithium chloride is also used to suppress enterococci, which also have the ability to hydrolyze esculin. Growth of the associated bacteria is largely inhibited by the addition of nalidixic acid and acryllaquine hydrochloride (MS131I).

Package size is available: 500 g

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Fraser Broth Base, 500 gr

  • 2,000.00 грн

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