Glucose-phosphatic broth - a liquid nutrient medium with a strong buffer properties. The main use of environment - the cultivation of bacteria for the test of Fogasa-Proskauer. Bacterial growth is accompanied by a diffuse clouding of the medium, and the bacteria produce acetoin after the addition of reagent (α-naphthol, potassium hydroxide) stain culture liquid red. If it does not stir produces the characteristic "red ring" at the top of the tube.
Purpose: Foges Proskoer test and reaction with methyl red
Principle of action: The buffer supports the weakly acidic pH of the medium. Glucose is a source of carbohydrates, from which the acid is produced, which is necessary for the reaction with methyl red. Also, during the fermentation of glucose, some microorganisms accumulate acetone in the form of by-products. The reaction of Foges-Proskauer detects the presence of acetone in a culture fluid
Peptone is femtomatic - 7 g / l
Glucose - 5 g / l
Sodium Hydrophosphate - 2 g / l
Potassium dihydrogenphosphate - 3 g / l
Instructions for use:
1. Dissolve 17 g of medium while heating in 1 liter of distilled water.
2. Boil 2-3 min until complete dissolution of the components
3. Pour 5 ml into the test tubes and autoclate 110 C for 20 minutes.
PH level: 6.9-0.2 ° C at 25 ° C
Delivery time: 5 р.д.
Glucose-phosphatic broth, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days