Vi-Salmonella TPHA - Reagent Kit for Detecting Antibodies
to Vi-antigen Salmonella in the reaction of passive hemagglutination
"Reagent kit for determining antibodies to Vi-antigen of Salmonella in the reaction of passive hemagglutination" "Vi-Salmonella RPGA" is designed to detect antibodies to Vi-antigen of the causative agent of typhoid fever in the reaction of passive hemagglutination (RPGA), in the format of qualitative and semi-quantitative tests.
"Vi-Salmonella RPGA" is indicated for use in the detection of bacterial carriers, including among food workers. When examining individuals who had been in contact with patients with typhoid fever or S. typhi carriers. To assess the effectiveness of vaccination against typhoid fever.
In addition to typhoid fever, the detection of antibodies to Vi-antigen is possible with salmonellosis caused by Salmonella paratyphi C and Salmonella dublin, which are also capable of producing Vi-antigen.
MAJOR CONSUMER CHARACTERISTICS
· The kit is designed for carrying out 64 analyzes, including controls, in the format of a qualitative (screening) method or on 12 analyzes, including controls, in the format of titration of serums (semi-quantitative method).
· For the study, samples of serum (plasma) of blood in a volume of 10 μl are taken.
· Color indication of sample entry.
· All kit components are ready to use.
· The total length of the incubation stages of the analysis is 2 hours.
PRINCIPLE OF THE METHOD
The method is based on the reaction of passive (indirect) hemagglutination. In the presence of antibodies to the Vi-antigen, hemagglutination of erythrocytes sensitized with the Vi-antigen is observed. This leads to the formation on the bottom of the wells of the tablet "umbrella" of the settled red blood cells. In the absence of antibodies to the Vi-antigen, hemagglutination of sensitized erythrocytes does not occur, and the erythrocytes form a "point".
The shelf life of the set is 18 months. Set expired application is not subject to.
STORAGE AND TRANSPORTATION
In the packaging of the manufacturer at a temperature of from 2 to 8 oC. Freezing is not allowed.
Storage after opening of the kit components - for the entire shelf life at a temperature of from 2 to 8 ° C.
At a temperature of 2 to 8 oC. Freezing is not allowed. Transportation is allowed at a temperature of from 9 to 25 ° C for 10 days.
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