TINSDALE AGAR BASE

Used for selective selection and differentiation of Corynebacterium diphtheria.

Composition g / l

Peptone of meat 20,00

L-cystiine 0.24

Sodium chloride 5.00

Sodium thiosulfate 0.43

Agar-agar 15.00

Appearance:

A bottle of 500 g of dry medium.

Method of cooking:

Mix 40.7 g of dry medium in 900 ml of distilled water. The mixture is boiled to the full

dissolving ingredients. Bring the volume of the liquid to 1000 ml, check the pH and, if necessary,

adjust the value to 7.4 ± 0.2, using a 10% solution of NaOH or a 10% solution of HCl.

Sterilize in an autoclave at 121 ° C for 15 minutes.

Density of the finished medium:

The medium responsible for the density of 1.5% agar gel is formed.

Color and transparency of the finished environment:

The ready environment has a light amber color, transparent, lightly opalestsuetsya if in cups

Petri then formed a gel.

Acidity of the medium:

At 25 ° C, an aqueous solution (4.0% w / v) pH of 7.4 ± 0.2.

Principle and evaluation of the result

 Used for selection and differentiation of species of Corynebacterium diphtheria.

• Peptone from meat provides the necessary nitrogen compounds.

 L-cystine and sodium thiosulfate are responsible for determining H2S.

 The added potassium tellurite is suppressed by the growth of all gram-positive bacteria and

most microorganisms that are part of the microflora of the upper respiratory tract.

 After inoculation of the medium by the test sample, place it in the incubator at 40-48

hours at a temperature of 35-37 ° C.

TECHNICAL PASSPORT

www.sanimed.com.ua 44265 May 2017 2

Results:

Microorganism Streams (ATCC) Rising Color

Corynebacterium diphtheriae

-gravis

-intermediaries

-mith

abundant Black-

brown with a halo

Streptococcus pyogenes (19615) abundant Black-

brown without halo

Klebsiella pneumoniae (13883) suppressed --- (19435) abundant