TCBS Agar are recommended for selective isolation and cultivation of cholera and food poisoning vibrio.

Composition g / l

Proteasapepton 10.00

Yeast extract 5,00

Thiosulfate sodium 10.00

Citrate sodium 10.00

Deoxycholatum sodium 2,00

Bile salts 8,00

Sucrose 20.00

Citrate of iron 1,00

Sodium Chloride 10.00

Bromotymol blue 0.04

Timolol blue 0.04

Agar 15.00

Appearance: A bottle of 500 g of dry medium.

Method of preparation: Mix 89.00 g of dry medium in 1000 ml of distilled water. Boil the mixture until the components are completely dissolved, check the pH and, if necessary, adjust the value to 8.6 ± 0.2, using a 10% solution of NaOH or a 10% solution of HCl. DO NOT AUTHORIZE THE ENVIRONMENT. Cool to 50 ° C and pour into sterile Petri dishes. Density of the prepared medium: A medium corresponding to density of 1.5% agar gel is formed. Color and transparency of the finished medium: The finished medium has a blue-green color, slightly opalescent, if a gel is formed in Petri dishes. Acidity of the medium: At 25 ° C, aqueous solution (8.9% w / v) pH 8.6 ± 0.2.

Principle and evaluation of the result:  It is recommended for isolation and growth of different types of Vibrio.  Peptone and extract of yeast, providing a connection with nitrogen  Bile salts that inhibit the growth of gram-positive bacteria  Sodium thiosulfate is a source of sulfur and together with an iron quote forms an indicator system for the production of hydrogen sulfide 2  Sucrose is a source of fermenting carbohydrates. Bromotymol blue and thymol blue are pH indicators. The alkaline pH value contributes to the allocation of cholera vibrio. Given the fermentation of sucrose, its colonies in this medium have a yellow color. The same color (yellow) colonies form Vibrio alginolyticus. Vibrio parahaemolyticus does not ferment sucrose and therefore forms blue-green colonies, like Vibrio vulnificus. Some strains of pseudomonads and aeromonads can give blue-green colonies, but in general, this medium is highly selective and colonies on it, as a rule, form only vibrios.  After inoculation of the medium with the test sample, place it in an incubator for 24-48 hours at a temperature of up to 35-37 ° C. Results: Microorganism Streams (ATCC) Growth Color Vibrio cholerae (15748) abundant yellow Vibrio parahaemolyticus (17802) abundant blue Escherichia coli (25922) suppressed - Proteus vulgaris (13315) suppressed - Streptococcus faecalis (29212) suppressed - Recommended storage conditions: Store in a dry environment at a temperature of 10-18 ° C, ready medium - at 2-8 ° C. Shelf life: 5 years during the period indicated on the label, under appropriate storage conditions.

Packaging: Plastic pot 500 gr