Citrate agar has the carbon source in the form of citrate. By the formation of alkaline waste products, phenol red allows detecting fermentation of citrate. In contrast to the "hungry" mineral Simmons, Christensen includes a certain amount of glucose, yeast extract and cysteine. The main source of carbon is still citrate and redness of the environment evidence of its fermentation.

Purpose: Differentiation of enterobacteria on the ability to ferment citrate.

Principle of action: In the process of fermentation of citrate, alkaline products are formed that change the color of the indicator and the medium becomes red.

Storage

Agar microbiological - 11,5 g / l,

sodium chloride - 4.5 g / l,

sodium citrate - 3 g / l,

potassium dihydrogenphosphate-1 g / l,

yeast extract - 0.5 g / l,

cysteine ​​hydrochloride - 0.1 g / l,

glucose - 0.2 g / l

phenolic red - 0,012 g / l.

Instructions for use

1. Dissolve 21 g of the medium when heated to 1 liter of purified water.

2. Boil for 2-3 minutes, until the components are completely dissolved, poured into test tubes.

3. Autoclave for 20 minutes at 112 ° C. 4. After autoclaving, the medium is slipped, leaving a column about 15-20 mm.

The pH is 6.5 ± 0.2 at 25 ° C

The information is valid: 04/23/2018