Malonat agar - the nutrient medium where the carbon source is malonate sodium, and a source of nitrogen - ammonium chloride. Other sources are absent. If the bacteria are able to ferment this substrate, they will produce alkaline waste products also bronchi ology blue, which is part of the environment, changes color from green to blue.

Purpose: Differentiation of microorganisms by the ability to ferment malonate

Principle of action: Malonate is the only environment vuleod. Ammonium chloride is a carbon source. In the environment there is an indicator - bromotymol blue, which reacts to fermentation of malonate and changes the color from green to blue.

Storage:

Agar microbiological - 8,5 g / l

Sodium malonate - 3 g / l

Ammonium chloride - 2 g / l

Potassium Hydrophosphate - 1 g / l

Yeast extract - 0.5 g / l

Bromotymol blue - 0,043 g / l

Instructions for use:

1. Dissolve 15 g of medium when heated in 1 liter of purified water.

2. Boil 2-3 minutes, until the components are completely dissolved.

3. Autoclaving for 20 min at 112 ° C

PH level: 6.7-0.2 ° at 25 ° C

Delivery time: 5 d.