Lewenstein-Jensen medium base is used for the preparation of medium for isolation and cultivation of mycobacteria.

The preparation is carried out as follows: the preparation of sterile egg emulsion, preparation of a basis environment the mixing of the emulsion and the basics, coagulation the finished medium.

As the inhibitor is present on the basis only of malachite green to the environment can add antibiotics to suppress the accompanying microflora. The kit consists of a powder for the preparation of bases and an appropriate amount of glycerol.