Colombian agar is recommended for the isolation of exciting pathogenic microorganisms (streptococci, staphylococci,

pneumococci).

Composition: g / l

Special peptone 23.00

Corn Starch 1.00

Sodium Chloride 5.00

Defibrillated ram blood 50 - 70 ml

Agar-agar 15.00

Appearance:

A pack of 10 cups of Petri x 18 ml medium ready to use.

Density of the finished medium:

The medium responsible for the density of 1.5% agar gel is formed.

Color and transparency of the finished environment:

The finished medium has a cherry-red color, slightly opalescent, if in Petri dishes then

gel is formed.

Acidity of the medium:

At 25 ° C, an aqueous solution (4.4% w / v) pH of 7.3 ± 0.2.

Principle and score of the result:

 It is recommended to isolate pathogenic microorganisms (streptococci,

staphylococci, pneumococci).

 When mixing with special additives, the environment can be used for

Selective selection of Brucella and Campylobacter.

 Special peptone provides the required amount of nitrogen, carbon and vitamins. Chloride

sodium supports the osmotic balance of the environment. Corn starch is one of

energy sources, and also neutralizes toxic metabolites.

 After inoculation of the medium by the test sample, place it in the incubator on

48 hours at a temperature of 35-37 ° C.

Results:

Microorganism Streams (ATCC) Growth

Staphylococcus aureus (25923) abundant

Staphylococus epidermidis (12228) abundant

Mycoplasma pneumoniae (15531) abundant

Streptococcus pneumoniae (6303) abundant