Acetate agar is used for differentiation according to the ability to ferment acetate. Due to the fact that acetate in the medium composition is the sole source of carbon, bacterial growth, are not capable of disposal, is suppressed. Bacteria that fermented it in the course of their life produce an alkaline solution which changes the medium color from green to blue. Samples cultured at 37 ° C, the final result is evaluated after 72 hours, although usually within 24 hours the reaction is already taking place. The duration of cultivation depends on the strain that you analyze.
Purpose: Differentiation of enterobacteria by the ability to ferment acetate.
Principle of operation: Inthe alkaline fermentation of acetate produced products that change color indicator in seredovyi from green to blue.
Storage:
Agar microbiological - 15 g / l
Sodium chloride - 5 g / l
Sodium acetate - 2 g / l
Potassium dihydrogenphosphate - 1 g / l
Ammonium hydrophosphate - 1 g / l
Bromotymol blue is 0.08 g / l
Instructions for use:
1. Dissolve 24 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes until the components completely dissolve
3. Autoclaving at 121 C for 20 min
PH level: 6.8-0.2 ° C at 25 ° C
Delivery time: 5 р.д.
ACETATE AGAR , 100 gr
- Brands ООО "Фармактив" Украина
- Product Code: 100 гр
- Availability: 2-3 Days
-
308.00 грн
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