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LISTERIA OXFORD AGAR BASE

It is recommended for the selection of Listeria species from pathological material

Composition g / l

Peptone special 23.00

Lithium chloride 15.00

Sodium chloride 15.00

Starchy corn 1,00

Agar-agar 10.00

Iron ammonium citrate 0,50

Esculin 1.00

Appearance:

A bottle of 500 gs. Medium.

Method of cooking:

27.75 g of dry medium, dissolve 1000 ml of distilled water. Mixes to the full

dissolution of the ingredients. , check the pH and, if necessary, adjust the value to 7,0 ± 0,2,

using 10% NaOH solution or 10% HCl solution. Sterilize in an autoclave at 121 ° C for

15 minutes.Heat to 500C and asepticallyaddissolved in 50% ethanol content of the vial from

a selective additive for the separation of the lysterics.

Density of the finished medium:

The medium responsible for the density of 1.0% agar gel is formed.

Color transparency of the finished medium:

The finished medium is dark-amber color, a slight precipitate, slightly opalescent.

Acidity of the medium:

At 25 °, a bulk solution (5.55% w / v) pH 7.0 ± 0.2.

Principle and score of the result:

 Used to isolate Listeriamonocytogenes from clinical specimens and specimens

food products.

 Lithium chloride and antibiotics inhibit the growth of gram-negative microflora and overwhelming

parts of the gram-positive flora of Listeriamonocytogenes, hydrolyses saculin on esculin and

glucose When reacting with iron citrate, esculental forms a brown-black complex,

which leads to the darkening of the environment around the colonies.

 The method of allocating the liters depends on the type of the material being studied. To all

Samples are recommended for selective and cold enrichment. Feces and other biomaterials

homogenize in 0.1% peptone water and 0.1 ml of the resulting suspension are sown on

Oxford agar, or in the broth for selective enrichment of the listeria, are incubated at

30S for 7 days, after which they are sown on a selective medium.

 After inoculation of the medium by the test sample, it is placed in the incubator

for 24-48 hours at a temperature of 35-37 ° C.

Results:

Microorganism Streams (ATCC) Growth Hydrolysis of the esculin

Listeriamonocytogenes (19117) abundant +

Listeriamonocytogenes (19111) abundant +

Staphylococcus aureus (25923) good -

Enterococcusfaecalis (29212) Inhibition -

Enterococcus tuberculosis (10541) inhibition -

Bacillus tuberculosis (6633) inhibition -

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LISTERIA OXFORD AGAR BASE, 500 gr

Brands Micromaster, Индия
Product Code: 500 гр
Availability: 2-3 Days

3,200.00 грн

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LISTERIA OXFORD AGAR BASE, 500 gr

3,200.00 грн

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