The Gissa medium with glucose is used to differentiate microorganisms by the ability to ferment sugar and alcohols. The medium indicator, bromocresol purpura, when fermenting carbohydrate (alcohol) and acid separation, changes the color from violet to yellow, indicating the ability of bacteria to ferment this carbohydrate (alcohol). The medium is semi-rigid, crop is carried out by the method of "puncture" by a microbiological loop. The test tubes are incubated at 37 ° C. for 24 hours, after which the color change is assessed.
Purpose: Differentiation of microorganisms by the ability to ferment carbohydrates
Principle of action: In the environment there is an indicator - bromocresol purple, which reacts to the fermentation of carbohydrates by changing the color from violet to yellow. If coloration remains violet, carbohydrate fermentation does not occur.
Glucose - 3.78 g / l
The peptone is enzymatic - 3.7 g / l
Agar microbiological - 2.5 g / l
Sodium chloride - 3 g / l
Sodium hydrophosphate - 0.5 g / l
Instructions for use:
1. Dissolve 13.5 g of medium when heated in 1 liter of purified water.
2. Boil 2-3 minutes, until the components are completely dissolved.
3. Autoclate for 20 minutes at 110 ° C
PH level: 8.2-0.2 ° at 25 ° C
Delivery time: 5 d.
Information updated: 02.03.2018
Gissa's medium with glucose, 250 gr
- Brands ООО "Фармактив" Украина
- Product Code: 250 гр
- Availability: 2-3 Days