Nutrient medium GNK agar is intended for the isolation and cultivation of gonococcus in the study of infected material from patients with inflammatory diseases of the urinary and other organs, as well as for the cultivation of museum strains.
It is a hygroscopic fine powder of light gray color.
Pancreatic hydrolyzed fish meal, yeast extract, hydrochloric acid hydrolyzed casein, growth stimulator of hemophilic microorganisms, glucose, agar.
The preparation in the amount necessary for preparing a specific nutrient batch is stirred in 1 liter of distilled water, sterilized by autoclaving at a temperature of 112 ° C for 30 minutes. Cooled to a temperature of 45-50 oC. To suppress the growth of the concomitant gonococcus bacterial flora, antibiotics are added: polymyxin-B sulfate 20,000 U / l and lincomycin hydrochloride 0.002 g / l, previously diluted in sterile saline. Medium with antibiotics should be used simultaneously with medium without antibiotics (one tube with medium with antibiotics, the other - without them), in view of the presence of antibiotic-sensitive strains of gonococcus. To improve the stability of the results, it is possible to add 100 ml of cattle blood serum to the medium. Mix thoroughly, pour into sterile Petri dishes 20 ml or 5 ml tubes and mow.
The finished medium is clear and brown. After autoclaving in the medium a small sediment is allowed
Prepared medium poured into Petri dishes or sterile tubes can be used for 14 days provided it is stored at a temperature of 2-8 oC.
pH 7.3 ± 0.2.
The combination of the components that make up the medium ensures the growth of gonococcus culture and inhibits the growth of extraneous microflora.
GNK agar, 250 gr
- Brands Micromaster, Индия
- Product Code: 250 гр
- Availability: In Stock
Tags: GNK agar