ПОМОЖЕМ СОСТАВИТЬ БЮДЖЕТ НА ТОРГИ ПО ПРЕДМЕТУ ЗАКУПКИ: ПИТАТЕЛЬНЫЕ СРЕДЫ, ДИСКИ С АНТИБИОТИКАМИ, СЫВОРОТКИ ДИАГНОСТИЧЕСКИЕ, БЫСТРЫЕ ТЕСТЫ т. 099-207-04-66 Дмитрий Escherichia coli O type, 1 ml, SSI Diagnostica, Denmark, price, buy
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  • Escherichia coli O type, 1 ml, SSI Diagnostica, Denmark

Escherichia coli O type, 1 ml, SSI Diagnostica, Denmark

Application

Immune serum of E. coli from Statens Serum Institut is intended for screening of  E. coli strains. It is important to confirm the result with immune serum  for use with boiled cultures.

O1, O2, O3, O4, O5, O6, O7, O8, O9, O10, O11, O12, O13, O14, O15, O16, O17, O18ab, O18ac, О19, O20, O21, O22, O23, O24, O25, O26, O27, O28ab, O28ac, O29, O30, O32, O33, O34, O35, O36, O37, O38, O39, O40, O41, O42, O43, O44, O45, O46, O48, O49, O50, O51, O52, O53, O54, O55, O56, O57, O58, O59, O60, O61, O62, O63,O64, O65, O66, O68, O69, O70, O71, O73, O74, O75, O76, O77, O78, O79, O80, O81, O82, O83, O84, O85, O86, O87, O88, O89, O90, O91, O92, O93, O95, O96, O97, O98, O99, O100, O101, O102, O103, O104, O105, O106, O107, O108, O109, O110, O111, O112ab, O112ac, O113, O114, O115,O116, O117, O118, O119, O120, O121, O123, O124, O125abc, O126, O127, O128abc, O129, O130, O131, O132, O133, O134, O135, O136, O137, O138, O139, O140, O141, O142, O143, O144, O145, O146, O147, O148, O149, O150, O151, O152, O153, O154, O155, O156, O157,O158,O159,O160, O161, O162,O163,O164,O165, O166,O167,O168,O169,O170, O171, O172, O173,O174,O175,O176,O177,O178, O179, O180, O181, O182 ,O183, O184, O185, O186, O187, O188

 Description

Immune serum of E. coli is grown in rabbits. Immune serums are supplied in vials of 1 ml (sodium azide for storage).

Principle

When the bacterial culture is mixed with a specific immune serum directed against the components of the surface of the bacterium, the cells are joined together by antigen-antibody bonds to form aggregates (agglutination). This, as a rule, is visible to the naked eye in the form of lumps in the suspension.

Materials that are needed but not included in the set

Agar medium (for example, meat peptone agar)

Inoculation loops or toothpicks

Pipettes

Object glass

Physiological solution, pH 7.4

The incubator is set to 37 ° C

Procedure

General

The physiological solution is used as a negative control, and it should be negative.

If the negative control is positive (agglutination), the strain automatically agglutinates.

Agglutination on a slide with an immune serum K9

The colon is grown overnight at a temperature of 37 ° C in a suitable agar medium that does not slow down the mobility.

1. Apply a small drop of immune serum (approximately 20 μL) to the slide glass.

2. Add culture from one colony to a drop of immune serum and mix well. The amount of culture should be sufficient to give a distinct, muddy-colored color. Use an inoculation loop or toothpick.

3. Tilt the glass forward and backward for no more than 5-10 seconds.

4. The reaction is read by the naked eye, holding the glass in front of a light on a black background (indirect light).

5. Positive reaction is considered as visible agglutination. Negative reaction is the preservation of mud-milk homogeneity. Late or weak agglutination should be considered a negative result.

6. The positive result should be confirmed by the positive reaction of the immune serum O104.

Storage and shelf life

Store at 2-8 ° C in a place protected from light. The expiration date is on the package. Occasionally, after prolonged storage, turbidity is observed due to precipitation of lipoproteins. Precipitations and / or contamination can be removed by centrifugation (10,000 g) followed by sterilization filtration (0.22 μm).

Support for defining types

Intestinal stems that can not be identified can be sent for further study to the International Center for the Ezergy (WHO), 5 Boulevard Orestads, DK-2300 Copenhagen S, Denmark. Please ask questions about costs.

Delivery time: 5 days

Information updated: 03.03.2018

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Escherichia coli O type, 1 ml, SSI Diagnostica, Denmark

  • 2,500.00 грн


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