ПОМОЖЕМ СОСТАВИТЬ БЮДЖЕТ НА ТОРГИ ПО ПРЕДМЕТУ ЗАКУПКИ: ПИТАТЕЛЬНЫЕ СРЕДЫ, ДИСКИ С АНТИБИОТИКАМИ, СЫВОРОТКИ ДИАГНОСТИЧЕСКИЕ, БЫСТРЫЕ ТЕСТЫ т. 099-207-04-66 Дмитрий Syphilis RPR-test, 100 determinations, price, buy, ukraine
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Syphilis RPR test

1. PURPOSE

For serodiagnosis of syphilis and control of the results of his treatment.

2. CHARACTERIZATION OF THE KIT

2.1. Operating principle

The test is based on the interaction of the cardiolipin antigen with the corresponding antibodies (reactants) in the serum or plasma of the patient with syphilis. To increase the apparent differences between the positive and negative reaction results, carbon particles were added to the antigen. If there are reactants in the sample, black aggregates are visible, visible to the naked eye. In the absence of reactants, flocculation does not occur, and the coal suspension remains homogeneous.

2.2. Kit Contents

Reagent 1- RPR-antigen - aqueous 0.02% suspension of coal particles sensitized with a mixture of cholesterol, cardiolipin, lecithin; a homogeneous suspension from gray to black color, when stored, separated into a black precipitate and a clear colorless supernatant.

Reagent 2- positive control serum (K +) - inactivated, contains antibodies to cardiolipin antigen in a titer of at least 1: 4 (titer is indicated in the passport of each series); transparent colorless or light yellow liquid.

Reagent 3- negative control serum (K-) - inactivated; transparent colorless or light yellow liquid.

The delivery set also includes reaction cards.

The kit is available in two versions:

Set No. 1 (calculated for 100 determinations);

Set No. 2 (calculated for 500 determinations).

Component name

Set No. 1

Set No. 2

Reagent 1 (RPR antigen)

1 f. (2.0 ml)

5 fl. (2.0 ml each)

Reagent 2 (K +)

1 f. (1.0 ml)

2 fl. (1.0 ml each)

Reagent 3 (K-)

1 f. (1.0 ml)

2 fl. (1.0 ml each)

Reaction cards

10 pieces.

50 pcs.

3. ANALYTICAL AND DIAGNOSTIC CHARACTERISTICS OF THE KIT

The kit is designed to hold 100 or 500 determinations, including control samples.

4. PRECAUTIONS

The kit is biologically safe, but the samples should be treated as potentially infectious materials.

5. EQUIPMENT AND MATERIALS

- Shaker orbital (any brand with an amplitude of oscillations of 10-20 mm).

- Micropipette automatic, with tips at 50 μl.

- Physiological saline - 0.9% solution of sodium chloride in purified water (distilled or deionized).

6. ANALYZED SAMPLES

Native or heated serum or plasma. Do not use haemolysed, chyle or germinated specimens. Serums intended for study can be stored up to 7 days at a temperature of 2-8 ° C or up to 3 months. at a temperature of -20 ° C.

7. CONDUCTING ANALYSIS

7.1. Preparation of test samples

All reagents are maintained for 15-20 minutes at room temperature prior to the start of the assay.

The suspension of the RPR antigen is shaken until uniform.

7.2. Carrying out the analysis

Qualitative definition

1. Pipette 50 microliters (one drop each) of K +, K- and test specimens into disposable card cells (one cell per sample) with a micropipette. Spread the liquid over the entire surface of the cell with the tip of a micropipette. For each sample use a separate tip.

When working with cards, do not touch the cells with your fingers!

2. Apply 20 μl of RPR antigen to all cells. Place the cards on the platform of the orbital shaker and rotate in a horizontal plane for 8 minutes at a speed of 180 rpm. Immediately after this, record the results.

The investigation of K + and K- must be carried out with each series of samples under study.

Semiquantitative definition (definition of a title)

It is carried out only with samples that have given a positive reaction.

1. Prepare on a one-time card serial double dilutions of the investigated samples and K + - from 1: 2 to 1: 1024. Use a separate card for each sample.

When working with cards, do not touch the cells with your fingers!

Apply 50 μl (one drop) of physiological solution to all test cells. Then add 50 μl (one drop) of the first test sample to cell No. 1 of the first card and mix with saline. After this, select 50 μl (one drop) of diluted 1: 2 sample from cell No. 1, transfer to cell No. 2 and mix. Repeat the procedure until the last dilution (1: 1024) is obtained in cell No. 10. Using the tip of the micropipette, distribute the droplets over the entire surface of the cells, moving from the larger dilution to the smaller.

Apply 20 μl of RPR antigen to all cells. Place the cards on the platform of the orbiting rotator and rotate in a horizontal plane for 8 minutes at 180 rpm. Immediately after this, record the results, starting with K +.

Unused reagents after the first opening of packages may be stored in tightly closed vials at a temperature of 2 to 8 ° C until the expiration date of the kit.

Unpacked bags with cards should be stored at room temperature.

 8. REGISTRATION AND ACCOUNT FOR RESULTS

Qualitative definition

Positive reaction - large, medium or small aggregates are visible in the sample.

Negative reaction - visibility

Delivery time: 5 days

The information is relevant to: 03.02.2018

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Syphilis RPR-test, 100 determinations

  • Brands ЭКОлаб
  • Product Code: 100 определений
  • Availability: 2-3 Days
  • 1,000.00 грн


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