Indicator paper systems for identification of microorganisms.
Set # 1 for identification of vibrios
Form of issue
Issue in a set of 13 tests: 11 vials with NIB-disks, and 2 tubes with NIB-strips.
Composition
The indicator paper systems (NIB) of the microorganism identification day (diagnostic kit) are discs or strips of chromatographic paper containing certain amounts of a substrate in combination with an indicator stabilized by a film-forming coating - polyvinyl alcohol.
NIB is released in a set of 13 tests: 11 vials with NIB-disks (NIB with glucose, lactose, sucrose, mannose, arabinose, mannitol, inositol, lysine, ornithine, arginine, for determination of urease) and 2 tubes with NIB-strips for the determination of oxidase and indole). NIB-disks are produced in vials, NIB-strips in test tubes in a cardboard bundle with instructions for use.
Set No. 1 provides 50 tests.
Indications for use
Identification and differentiation of microorganisms of the genus Vibrio by biochemical activity.
Dosage regimen and method of administration
Objects of research in sanitary and clinical microbiology.
CONDUCTING ANALYSIS
The basic requirements for working with a set of reagents "Indicator paper systems for the identification of microorganisms. Set No. 1 for identification of vibrios ":
- studies are conducted with a pure culture, as well as with individual colonies with
nutrient agar for isolation and cultivation of vibrios;
- immersion of discs in test tubes is made with burnt tweezers;
- a criterion for the correctness of the reaction should be clear differences in the color of the
compared with the control (with the exception of amino acids, where in the test tube the weak
green color indicates a negative result, while in
control discs with amino acids are colored yellow);
-to obtain clear results, it is necessary to observe the temperature regime of the thermostat (37 ± 1) ° С, the pH of the nutrient media used and the treatment mode: dishes.
Determination of oxidase activity.
The culture grown on the surface of the nutrient agar for culturing microorganisms for 18-24 hours at a temperature of (37 ± 1) ° C is triturated with a platinum loop or glass rod on a test strip of NIB placed in a Petri dish. On one strip you can study 10-14 cultures (no more).
Determination of the utilization of carbohydrates and polyhydric alcohols.
In a test tube with 0.3 ml of sterile sodium chloride solution of 0.9% pH 7.3 ± 0.1, a complete loop of culture grown on the surface of nutrient agar for culturing microorganisms is suspended for 18-24 hours at a temperature of (37 ± 1) ° C, then immerse in a test tube NIB-disk with the appropriate carbohydrate or polyhydric alcohol. The medium in the test tubes becomes red as a result of the rapid diffusion of the indicator into it. If it is necessary to take into account gas formation, it is recommended to use a small lump of sterile absorbent cotton, which is placed in a test tube. As a control, NIB disks immersed in test tubes with sterile sodium chloride solution of 0.9% pH 7.3 ± 0.1 are used. The tubes are incubated at a temperature of (37 ± 1) ° C.
Definition of indole formation.
In a test tube with 0.3 ml of sterile sodium chloride solution of 0.9% pH 7.3 ± 0.1, a complete loop of the culture grown on the surface of the agar for culture of MIS organisms for 18-24 h at a temperature of (37 ± 1) ° C FROM. The strip for the determination of the nondola is folded along the line planned for it in half and plunged into the bottom of the tube with tweezers so that the long colorless end is immersed in the suspension of the culture, and the short colored: the end is above the surface of the suspension. As a control, use a strip placed in a test tube with sterile sodium chloride solution of 0.9% pH 7.3 ± 0.1. Incubate at a temperature of (37 ± 1) ° C.
Determination of urease activity.
In a test tube with 0.3 ml of sterile sodium chloride solution of 0.9% pH 7.3 ± 0.1, a complete loop of culture grown on the surface of nutrient agar for culturing microorganisms is suspended for 18-24 hours at a temperature of (37 ± 1) ° C, then a SIB disk with urea is dipped into this slurry. As a control, a NIB disk is used, placed in a test tube with sterile sodium chloride solution of 0.9% pH 7.3 ± 0.1. Incubate at a temperature of (37 ± 1) ° C.
Determination of decarboxylases of lysine, ornithine and arginine dehydrolase.
In a test tube with 0.3 ml of a sterile phosphate buffered saline solution pH 5.5 ± 0.2, a complete loop of culture grown on the surface of nutrient agar for culturing microorganisms for 18-24 h at a temperature of (37 ± 1) ° C, Then a NIB disk with one of the amino acids lysine, ornithine or arginine is immersed in this slurry and 0.2 ml of sterile vaseline oil is added. As a control, NIB disks with the appropriate amino acids are used, immersed in test tubes with a sterile phosphate buffered saline solution pH 5.5 ± 0.2 with the addition of 0.2 ml sterile vaseline oil. Incubate at a temperature of (37 ± 1) ° C.
Indicator paper systems for identification of vibrios
- Brands Агар ЮА
- Product Code: набор
- Availability: 2-3 Days
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7,000.00 грн