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  • BLOOD AGAR BASE, 500 gr
BLOOD AGAR BASE
Once the blood is added, you can use it to select and cultivate the nourishing ones
microorganisms
Composition g / l
Proteus for peptone 15.00
Yeast extract 5,00
Liver Extract 2.50
Sodium Chloride 5.00
Agar-agar 15.00
Appearance:
A bottle of 500 g of dry medium.
Method of cooking:
Mix 42.50 g of dry medium in 900 ml of distilled water. The mixture is boiled to the full
dissolve the ingredients, check the pH and, if necessary, adjust the values ​​to 7.4 ± 0.2,
using a 10% solution of NaOH or a 10% solution of HCl. Bring the volume of the liquid to 1000 ml and
Sterilize in an autoclave at 121 ° C for 15 minutes. The medium is cooled to 45-50 ° C and aseptically
mixed with sterile defibrillated blood / serum (7 vol%).
Density of the finished medium:
The medium responsible for the density of 1.5% agar gel is formed.
Color and transparency of the finished medium:
After the blood is added, the environment must have a cherry-red color and opalescents if in
Petri dishes formed gel.
Acidity of the medium:
At 25 ° C, an aqueous solution (4.0% w / v) pH of 7.4 ± 0.2.
Principle and score of the result:
 This medium is designed to isolate pathogenic microorganisms (streptococci,
staphylococci, pneumococci) without interference with their hemolytic activity.
 When mixing with special additives, the environment can be used for
Selective selection of Brucella and Campylobacter.
 Yeast extract, liver extract, and peptone provide nitrogen,
carbon and vitamins. Sodium chloride maintains the osmotic balance of the medium.
 After inoculation of the medium by the test sample, place it in the incubator on
48 hours at a temperature of 35-37 ° C.
Results:
Microorganism Streams (ATCC) Growth
Staphylococcus aureus (25923) abundant
Streptococcus agalactiae (13813) abundant
Streptococcus pneumoniae (6303) abundant

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BLOOD AGAR BASE, 500 gr

  • 2,600.00 грн


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