Bismuth sulfite agar is used for selective isolation and differentiation of Salmonella. The composition of the medium causes inhibition of the growth of extraneous microflora, and the grown colonies are greenish-brown. This stimulated the growth of Salmonella and their ability to the recovery of bismuth allows you to define them by blackening of the colonies and environment under them. Sowing is carried out by the surface method, the incubation is carried out at 37 ° C for 48 hours.

Purpose: Selective selection of salmonella.

Principle of action: The selective effect of the medium is achieved by bismuth citrate and diamond greens. Bacteria are capable of reducing sulfite to sulfide, coloring the colonies and the environment around them in black with a metallic shine (the result of binding of sulfide to bismuth and iron ions)

Storage:

Peptone is femtomatic - 17 g / l

Agar microbiological - 15 g / l

Glucose - 5 g / l

Sodium sulfate - 5 g / l

Sodium hydrophosphate - 4 g / l

Bismuth citrate - 2 g / l

Yeast extract - 1 g / l

Mora Salt - 1 g / l

Diamonds green - 0,025 g / l

Instructions for use:

1. Dissolve 50 g of medium when heated in 1 liter of purified water.

2. Boil 2-3 minutes until the components completely dissolve

3. Do not autoclate

PH level: 7,6-0,2 at 25 ° C

Delivery time: 5 р.д.