ПОМОЖЕМ СОСТАВИТЬ БЮДЖЕТ НА ТОРГИ ПО ПРЕДМЕТУ ЗАКУПКИ: ПИТАТЕЛЬНЫЕ СРЕДЫ, ДИСКИ С АНТИБИОТИКАМИ, СЫВОРОТКИ ДИАГНОСТИЧЕСКИЕ, БЫСТРЫЕ ТЕСТЫ т. 099-207-04-66 Дмитрий BILE ESCULIN AZIDE AGAR, 500 gr
Сыворотки
Диски с антибиотиками
Питательные среды
  • BILE ESCULIN AZIDE AGAR, 500 gr

Esophagal feces with sodium nitride are used to isolate and pre-identify fecal streptococci (enterococci).

Composition g / l

Hydrosis casein 17,00

Protozoopectone 3.00

Meat extract 5,00

Oxgall 10.00

Sodium chloride 5.00

Esculin 1.00

Iron ammonium citrate 0,50

Sodium azide 0.15

Agar-agar 15.00

Appearance:

Bottle of 500 g of dry medium:

Method of cooking:

Mix 56.65 g of dry medium in 1000 ml of distilled water. The mixture is boiled to

complete dissolution of ingredients, check pH and, if necessary, adjust the values ​​to 7.5 ± 0.2,

using a 10% solution of NaOH or a 10% solution of HCl. Sterilize in an autoclave at 121 ° C for

15 minutes.

Density of the finished medium:

The medium responsible for the density of 1.5% agar gel is formed.

Color and transparency of the finished medium:

The finished medium has an amber color, transparent, slightly opalestsuetsya, if in cups Petri then

gel is formed.

Acidity of the medium:

At 25 ° C, an aqueous solution (5.7% w / v) pH of 7.5 ± 0.2.

Principle and score of the result:

 The environment is selective for streptococcus group D and provides for their rapid growth.

 The environment is highly nutritious, because it contains a casein hydrolyzate, peptic grass of the animal

fabrics, proteosopepton, yeast extract and meat extract.

 Sodium azide inhibits the growth of gram-negative microorganisms, but allows you to grow fecal

streptococci.

 Esculin hydrolysis and bile tolerance allow to isolate and identify streptococci

Group D for 24 hours.

 After inoculation of the medium by the test sample, it is placed in an incubator at 18-24

hours at a temperature of 35-37 ° C.

Results:

Microorganism Streams (ATCC) Rising Color

Enterococcus faecalis (29212) abundant blackening

Streptococcus bovis (27960) abundant blackening

Staphylococcus aureus (25923) abundant -

Streptococcus pyogenes (19615) absent -

Proteus mirabilis (25933 abundant -

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BILE ESCULIN AZIDE AGAR, 500 gr

  • 3,250.00 грн


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