Esophagal feces with sodium nitride are used to isolate and pre-identify fecal streptococci (enterococci).
Composition g / l
Hydrosis casein 17,00
Protozoopectone 3.00
Meat extract 5,00
Oxgall 10.00
Sodium chloride 5.00
Esculin 1.00
Iron ammonium citrate 0,50
Sodium azide 0.15
Agar-agar 15.00
Appearance:
Bottle of 500 g of dry medium:
Method of cooking:
Mix 56.65 g of dry medium in 1000 ml of distilled water. The mixture is boiled to
complete dissolution of ingredients, check pH and, if necessary, adjust the values to 7.5 ± 0.2,
using a 10% solution of NaOH or a 10% solution of HCl. Sterilize in an autoclave at 121 ° C for
15 minutes.
Density of the finished medium:
The medium responsible for the density of 1.5% agar gel is formed.
Color and transparency of the finished medium:
The finished medium has an amber color, transparent, slightly opalestsuetsya, if in cups Petri then
gel is formed.
Acidity of the medium:
At 25 ° C, an aqueous solution (5.7% w / v) pH of 7.5 ± 0.2.
Principle and score of the result:
The environment is selective for streptococcus group D and provides for their rapid growth.
The environment is highly nutritious, because it contains a casein hydrolyzate, peptic grass of the animal
fabrics, proteosopepton, yeast extract and meat extract.
Sodium azide inhibits the growth of gram-negative microorganisms, but allows you to grow fecal
streptococci.
Esculin hydrolysis and bile tolerance allow to isolate and identify streptococci
Group D for 24 hours.
After inoculation of the medium by the test sample, it is placed in an incubator at 18-24
hours at a temperature of 35-37 ° C.
Results:
Microorganism Streams (ATCC) Rising Color
Enterococcus faecalis (29212) abundant blackening
Streptococcus bovis (27960) abundant blackening
Staphylococcus aureus (25923) abundant -
Streptococcus pyogenes (19615) absent -
Proteus mirabilis (25933 abundant -
BILE ESCULIN AZIDE AGAR, 500 gr
- Brands Micromaster, Индия
- Product Code: 500 гр
- Availability: 2-3 Days
-
3,250.00 грн
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Tags: BILE ESCULIN AZIDE AGAR, 500 gr