SET OF RESPONSES FOR DETERMINATION OF ACTIVITY OF ALANINAMINOTRANSPHERASE IN BLOOD SYROVISTS (KIN), ALAT

APPOINTMENT

The kit is used to determine the activity of alanine aminotransferase in human serum in clinical diagnostic, biochemical laboratories and research.

The set is designed for 60 macro, 125 semimicro or 250 micro values ​​(including blank samples)

Linearity should be maintained in the range from 0.1 μmol / (h) to

2.5 μmol / (hlm) (from 0.028 μc / l to 0.7 μc / l).

The coefficient of variation of determination is no more than 6%.

Storage set - at a temperature from plus 2 ° С to plus 8 ° С.

The warranty period is 12 months from the date of manufacture. The kit is intended for use in vitro by a trained technician.

PRINCIPLE OF THE METHOD

Due to the amination of 2-oxo-glutaric acid with L-alanine, which occurs under the action of alanine aminotransferase, L-glutamine and pyruvic acid are formed. The definition is based on the measurement of the optical density of 2,4-dinitrophenylhydrazones of 2-oxoglutaric and pyruvic acids in alkaline media. Since the pyruvic acid hydrazone has a higher molar extinction coefficient, there is a direct proportional dependence of the optical density of the reaction solution on the activity of the enzyme.

COMPOSITION OF THE SET

1. Sub-buffer solution AlAT-1 bottle with (50 ± 2) ml;

- phosphate buffer (0,100 ± 0,005) mol / l,

- D, L-alpha-alanine (0.20 ± 0.01) mol / L,

- 2oxo-glutaric acid (2.0 ± 0.1) mmol / liter.

2. Stop reagent - 1 bottle with (50 ± 2) ml;

- 2,4-dinitrophenylhydrazine (1,00 ± 0,05) mmol / liter

3. Gauge solution - 1 vial with (5,0 ± 0,5) ml;

- pyruvic acid sodium (2,0 ± 0,1) mmol / liter,

(220 ± 11) μg / ml (corresponding to 176 μg / ml of pyruvic acid)

4. Sodium hydroxide solution (4,0 ± 0,2) mol / L or dry - 1 bottle of (50 ± 2) ml (careful caustic!) Or (8,00 ± 0,32) g

DIAGNOSTIC CHARACTERISTICS

Aminotransferases catalyze the formation of glutamic acid from 2-oxoglutarate by the transfer of amino groups.

Normally, ALT is present in many tissues, but the highest concentrations are detected in the liver and kidneys.

The level of serum ALT increases with hepatitis and other liver diseases accompanied by necrosis of hepatocytes: infectious mononucleosis, cholestasis, cirrhosis, metastatic carcinoma of the liver, white fever and the appointment of various drugs such as opiates, salicylates or ampicillin 2.3.Clinical diagnosis should be based on the integration of clinical and laboratory data.

QUALITY CONTROL

It is recommended to use control serums with the values ​​determined by this method to control the reaction and measurement procedures. For example: Lionorm (Czech Republic), Biocont S (Russia), Filonorm or PhiloPat (Ukraine).

Each laboratory should establish its own internal quality control system.

Delivery time: 5 d.

Information updated: 02.03.2018