Agar Wilson-Blair is used to isolate clostridia. A slurry of sample is prepared, the solid phase is precipitated, and the supernatant portion of the suspension is introduced into the prepared Wilson-Blair sterile medium in test tubes at a temperature of about 70 ° C. The tubes are incubated at 37 ° C for 24-48 hours. Clostridia capable of reducing sulfur to convert sodium sulfite (which in high concentration is part of the Wilson-Blair agar) in hydrogen sulfide, which in turn reacts with iron ions, causing blackening of the medium.

In the analysis, aerobic microflora is removed, and part of vegetative cells that do not withstand the initial temperature of the medium.

Assignment of Wilson-Blair agar: Determination of sulfide-reductive ability of mesophilic anaerobes

The principle of the Wilson-Blair agar: When anaerobic cultivation of clostridia is able to restore sulfite to sulfide. Ions of sulfide are bound to iron to form a compound of black color.

Walshon-Blair agar composition:

Sodium sulfite - 20 g / l

Agar microbiological - 13 g / l

The peptone is enzymatic - 10 g / l

Glucose - 10 g / l

Sodium chloride - 5 g / l

Yeast extract - 1 g / l

Ferrum sulfate - 1 g / l

Instructions for using Wilson-Blair agar:

1. Dissolve 60 g of medium when heated in 1 liter of purified water

2. Boil for 2-3 minutes until complete dissolution of the components, pour into test tubes.

3. Autoclate for 20 minutes at a temperature of 112 C

PH level: 7.6-0.2 ° at 25 ° C

Delivery time: 5 d.

Information updated: 02.03.2018