Agar Klygler is a ready-made environment, due to phenol red has a red color. For analysis of Kigler agar, they are poured through the test tubes and cooled at an angle to form a bevel and a zone of a full column. Sowing on the Klyler's medium is carried out by surface dashed method and puncturing. After incubation at 37 ° C for 24 hours of culture, fermentation of glucose causes yellowing of the column, and those that ferment lactose - yellowing of the bevel. In this case, the synthesis of hydrogen sulfide is blackened in the column.

Purpose Differentiation of bacteria by the ability to ferment carbohydrates, urea and produce hydrogen sulfide.

Principle of action The medium is prepared in the form of sloped agar in test tubes. During fermentation of carbohydrates, the indicator in the environment changes the color. Depending on the part of the test tube in which the color of the medium occurred, it is possible to differentiate microorganisms. When the hydrogen sulfide forms, the black color of the ferric sulfide appears. Agar discontinuities indicate bacterial release of gas.

Storage

Peptone enzymatic-15g / l

Agar microbiological - 15g / l,

Lactose - 10 g / l,

Sodium chloride - 5,3 g / l,

Yeast extract - 3 g / l

Glucose-1 g / l

Sodium thiosulfate - 0.3 g / l,

Iron sulfate - 0.25 g / l,

Sodium pyrosulphite - 0,1 g / l

Phenol red - 0,03 g / l.

Instructions for use

1. Dissolve 50 g of the medium when heated to 1 liter of purified water.

2. Boil for 2-3 minutes, until the components are completely dissolved, pour 7 ml into the test tube.

3. Autoclave for 20 minutes at 112 ° C.

The pH is 7.4 ± 0.2 at 25 ° C

The information is valid: 04/23/2018